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For full induction, the plasmid requires a period of induction by subinhibitory concentrations of erythromycin. [3] Temperature sensitive. [3] Plasmid is a natural isolate from Staphylococcus aureus that confers resistance to macrolide, lincosamide and streptogramin type B 'MLS' antibiotics. [3] Bacillus Genetic Stock Center <-- B. Weisblum <-- B. Iordanescu. (ATCC staff) Restriction digests of the clone give the following sizes (kb): AccI--3.7; PstI--3.7. (ATCC staff) Medium is 1179 V Y medium. Hosts: B.subtilis BSU1E18, Bacillus, Staphylococcus aureus, B.subtilis BD170. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) |
