| 메모 |
This was constructed by inserting a 6.1 kb EcoRI/SalI HIS3-containing fragment from pGT2-Sc2605 into pBR322. The HIS3 fragment was originally isolated from the diploid strain A364a X H79-20.3alpha. [2] A general purpose integrating Saccharomyces cerevisiae/E.coli shuttle vector. [1] Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--9.0, 1.5; EcoRI--9.8. (ATCC staff) This construct is not stable when freeze-dried and is distributed from liquid nitrogen stock. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli K-12 r-m+, Saccharomyces cerevisiae, E.coli MM294, E.coli C600. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) |
