| 메모 |
This was constructed by inserting a 2.2 kb SalI/XhoI fragment containing LEU2 from pYe(Leu2)10 into the SalI site of pBR322. The 5' - 3' orientation of LEU2 is counterclockwise. LEU2, originating from strain X2180-1A, includes a region of Ty1-17. [2] A general purpose integrating Saccharomyces cerevisiae/E.coli shuttle vector. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli MM294, E.coli C600, S.cerevisiae SHY series. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) |
