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Created by Moore, July 1995, under contract with NCBI. This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 into the HindIII site of pBR322. The 5' - 3' orientation of URA3 is counterclockwise, with the SmaI site in 3' URA3 clone to the EcoRI site of pBR322. [2] HV2 vector when used with S. cerevisiae SHY series hosts (ATCC 44769 to ATCC 44772). (ATCC staff) A general purpose integrating Saccharomyces cerevisiae/E.coli shuttle vector. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli MM294, E.coli C600, S.cerevisiae SHY series. Related vectors: pBR322, YEp24. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) |