| 메모 |
A 1.1 kb HindIII fragment containing URA3 and a 2.2 kb SalI/XhoI fragment containing LEU2 were cloned into the HindIII and SalI sites, respectively, of pBR322. Unique restriction sites are in the 3' untranslated regions of URA3(SmaI) and LEU2(SalI). [2] Orientations are such that transcription from URA3 is counterclockwise and from LEU2 is clockwise. [2] A general purpose integrating Saccharomyces cerevisiae/E.coli shuttle vector. [1] Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).) |
